Visual Universitätsmedizin Mainz

Research projects

 

TICARDIO's program is designed to discover and validate new interactions in the crosstalk between vasculature- and blood-driven inflammatory and prothrombotic processes, and furthermore to identify diagnostic biomarkers and potential targets of intervention. The scientific projects are intertwined and require scientists with diversified skills to exploit a multitude of transgenic mice strains, available in the participating laboratories, with altered platelet, endothelial, or leukocyte phenotype, in combination with a broad range of new techniques in imaging (multi-photon, spinning-disc, and intravital microscopy; high-resolution ultrasound, high-end NMR spectroscopic imaging), flow cytometry, quantitative shot-gun and targeted (phospho)proteomics with high-throughput protein profiling using the newest Orbitrap mass spectrometers, omics (genomics, lipidomics, transcriptomics), exclusive multiplex microfluidic assays for ex vivo blood flow studies, providing environments that mimic the inflamed and thrombotic vessel wall (vessel-on-a-chip), and various coagulation assays. These techniques are applied to in vitro and in vivo studies, using the newest experimental models of thrombosis and inflammation. Providing these diverging techniques lays beyond the possibilities of a single institute, but will be essential to decipher the complex deregulated interactions in blood and vessel wall, causing thrombo-inflammation.

 

 

Project 1: Formation mechanisms of endothelial extracellular vesicles (EV) in thrombo-inflammation.

Host: Center for Thrombosis and Hemostasis, Mainz (Germany)

Supervisory team: primary: Prof. Dr. Katrin Schäfer (CTH); secondary: Prof. Dr. Chritophe Dubois (AMU); teriary: Dr. Stefan Kauschke (Boehringer Ingelheim)

 

 

Project 2: Converging and distinct roles of coagulation factors (F) XI and XII in vascular thrombo-inflammation

Host: Center for Thrombosis and Hemostasis, Mainz (Germany)

Supervisory team: primary: Prof. Dr. Philip Wenzel (CTH), secondary: Dr. Judith Cosemans (CARIM), tertiary: Dr. Stefan Heitmeier (Bayer)

 

 

Project 3: Blood coagulation and vascular protease-activated receptors to steer vascular remodeling.

Host: School for Cardiovascular Disease in Maastricht, CARIM (Maastricht, Netherlands)

Supervisory team: primary: Prof. Dr. Leon Schurgers (CARIM), secondary: Prof. Dr. Wolfram Ruf (CTH), tertiary: Dr. Stefan Kauschke (Boehringer Ingelheim)

 

 

Project 4: Vessel wall-driven platelet and neutrophil activation.

Host: Aix-Marseille Université (Marseille, France)

Supervisory team: primary: Prof. Dr. Christophe Dubois (AMU), secondary: Prof. Dr. Leon Schurgers (CARIM), tertiary: Dr. Stefan Kauschke (Boehringer Ingelheim)

 

 

Project 5: Senescence in vascular dysfunction and thrombo-inflammation.

Host: Aix-Marseille Université (Marseille, France)

Supervisory team: primary: Prof. Dr. Florence Sabatier (AMU), Prof. Dr. Katrin Schäfer (CTH), tertiary: Prof. Dr. Albert Sickmann (ISAS)

 

 

Project 6: Tissue factor pathway and extracellular vesicles in thrombo-inflammation.

Host: Center for Thrombosis and Hemostasis (Mainz, Germany)

Supervisory team: primary: Prof. Dr. Wolfram Ruf (CTH), secondary: Prof. Dr. Francoise Dignat-George (AMU), tertiary: Dr. Philippe Poncelet (BioCytex)

 

 

Project 7: Regulation of smooth muscle cell phenotype switching by tissue factor pathway inhibitor.

Host: School for Cardiovascular Disease in Maastricht (Maastricht, Netherlands)

Supervisory team: primary: Prof. Dr. Tilman Hackeng (CARIM), secondary: Philip Wenzel (CTH)

 

 

Project 8: Endothelial-derived inhibiting and activating pathways of platelets in thrombosis.

Host: Leibniz-Institut für Analytische Wissenschaften, ISAS (Dortmund, Germany)

Supervisory team: primary: Prof. Dr. Albert Sickmann (ISAS), secondary: Prof. Dr. Johan Heemskerk (CARIM), tertiary: PD Dr. Kerstin Jurk (CTH)

 

 

Project 9: Mechanisms of platelet and coagulation activation in inflammatory vessel-on-a-chip models.

Host: School for Cardiovascular Disease in Maastricht (Maastricht, Netherlands)

Supervisory team: primary: Prof. Dr. Johan Heemskerk (CARIM), secondary: PD Dr. Kerstin Jurk (CTH), tertiary: Prof. Dr. Albert Sickmann (ISAS)

 

 

Project 10: Multifaceted functions of extracellular vesicles in a thrombus and in thrombo-inflammation.

Host: Aix-Marseille Université (Marseille, France)

Supervisory team: primary: Prof. Dr. Francoise Dignat-George (AMU), secondary: Dr. Rory Koenen (CARIM), tertiary: Dr. Philippe Poncelet (BioCytex)

 

 

Project 11: Alternative splicing in the regulation of thrombo-inflammation.

Host: School for Cardiovascular Disease in Maatricht, CARIM (Maastricht, Netherlands)

Supervisory team: primary: Dr. Elisabetta Castoldi (CARIM), Prof. Dr. Pierre Morange (AMU), tertiary: Tim Beissert (TRON)

 

 

Project 12: Deregulated transcriptome 3’ end diversification affecting thrombo-inflammation.

Host: Center for Thrombosis and Hemostasis, CTH (Mainz, Germany)

Supervisory team: primary: Prof. Dr. Sven Danckwardt (CTH), secondary: Dr. Elisabetta Castoldi (CARIM)

 

 

Project 13: Epidemiolomics of thrombin generation in thrombo-inflammation.

Host: Aix-Marseille Université, AMU (Marseille, France)

Supervisory team: primary: Prof. Dr. Pierre Morange (AMU), secondary: Prof. Dr. Sven Danckwardt (CTH), tertiary: Dr. Stefan Heitmeier (Bayer)

 

 

Project 14: Multifaceted analysis of coagulant activity in venous thrombo-inflammation.

Host: Center for Thrombosis and Hemostasis, CTH (Mainz, Germany)

Supervisory team: primary: Prof. Dr. Philipp Wild (CTH), secondary: Dr. Henri Spronk/Prof. Dr. Hugo ten Cate (CARIM), Dr. Stefan Heitmeier (Bayer)

 

 

Project 15: Phenotyping atherothrombosis in patients at risk of cardiovascular disease.

Host: Schoolf for Cardiovascular Disease in Maastricht, CARIM (Maastricht, Netherlands)

Supervisory team: primary: Prof. Dr. Hugo ten Cate (CARIM), secondary: Prof. Dr. Philipp Wild, tertiary, Gillian Mimnagh (2M Engineering)

 

 

Further project details will follow soon.

 

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