Visual Universitätsmedizin Mainz

TRP X21 - Identification of prothrombin (F2) expressing cells in the hematopoietic compartment

Funding period: 01.01.2019 – 30.04.2020

Project Summary

The serine protease thrombin (F2) is involved in a number of physiological functions beyond hemostasis including angiogenesis, embryogenesis, tumorigenesis and inflammation. Serum levels of prothrombin derive from protein synthesis in the liver, yet expression of the prothrombin gene in a variety of other tissues has been demonstrated to occur during embryogenesis. While considerable insights have been obtained on how plasmatic thrombin exerts its crucial function in various pathologies, little is known about the (patho)physiological functions of extrahepatic prothrombin expression. Using a newly designed transgenic mouse model, we recently observed prothrombin expression in cells of hematopoetic origin.

In the project proposed here we are aiming to identify cell type(s) expressing prothrombin in the hematopoietic compartment. To this end, we will employ FACS sorting followed by luminometry based on purified cells obtained from the reporter mouse model in which endogenous prothrombin expression is tagged by luciferase reporters. Complementary, we will make use of a newly established F2 RNA-FISH technology, which will allow us to counter-validate extrahepatic prothrombin expression on a cellular level in intact murine organs.

Principal Investigators

Danckwardt, MD
Professor Sven Danckwardt, MD
Position: Professorship "Experimental Hemostasis and Laboratory Medicine", DZHK Scientist

06131 17-6041 or 17-2632
06131 17-5588
sven.danckwardt@unimedizin-mainz.de

PhD Probst
Hans Christian Probst, PhD
Position: Group leader, Institute of Immunology

06131/17-6198
hcprobst@uni-mainz.de