Visual Universitätsmedizin Mainz

Dr. Christian Freese

Wissenschaftlicher Mitarbeiter
Tel. +49 6131 39 23185
Fax 39 24743
 freesec@uni-mainz.de

 

 

CV

PostDoc - Institute of Pathobiochemistry, University Medical Center Mainz
2015 – present
Christian is working on autophagy which represents an important pathway for cellular protein and organelle degradation and whose disturbance seems to play a crucial role in cancer and several neurodegenerative disorders.

PostDoc - REPAIR-lab, University Medical Center Mainz
2011 - 2015
As a PostDoc he was supported by the ERA-Net Neuron project 'GINA' in which he investigated the behavior of neural and endothelial cells on biomaterial scaffolds.

PhD student - REPAIR-lab, University Medical Center Mainz
2008 - 2011
In 2008 he started as a PhD student at the Institute of Pathology at the University Medical Center of the Johannes Gutenberg University Mainz. The focus of his thesis was on the development of an in vitro blood-brain barrier model as well as on the interaction of nanoparticles with endothelial cells.

Diploma student - Institute of Biochemistry – Johannes Gutenberg University Mainz
2006 - 2007
Christian Freese finished his diploma in Biology at the Institute of Biochemistry at the Johannes Gutenberg University Mainz in 2007.


  

Methodologies

Complex cell culture model systems with focus on blood-brain barrier model system
Proteinbiochemistry (ELISA, WB, CAM-EIA)
Molecular biology (RT-PCR, PCR, cloning)
Isolation of primary human cells (brain, skin, umbilical vein)
Light/Fluorescence Microscopy, Laser confocal scanning microscopy, Life cell microscopy

 

 

Publications

2015
Extract of Caragana sinica as a potential therapeutic option for increasing alpha-secretase gene expression.
Florian Schuck, Ulrich Schmitt, Sven Reinhardt, Christian Freese, Ik-Soo Lee, Eckhard Thines, Thomas Efferth, Kristina Endres.
Phytomedicine, 2015: 22; 1027—1036.
doi:10.1016/j.phymed.2015.08.001

Enzyme-responsive nanocomposites for wound infection prophylaxis in burn management: in vitro evaluation of their compatibility with healing processes.
Verena Grützner, Ronald E Unger, Grit Baier, Lars Choritz, Christian Freese, Thomas Böse, Katharina Landfester, C James Kirkpatrick.
International Journal of Nanomedicine, 2015: 4111—4124.
http://dx.doi.org/10.2147/IJN.S81263

Identification of trichlormethiazide as a Mdr1a/b gene expression enhancer via a dual secretion-based promoter assay.
Sarina Schulze, Sven Reinhardt, Christian Freese, Ulrich Schmitt, Kristina Endres.
Pharmacology Research & Perspectives 02/2015; 3(1).
http://onlinelibrary.wiley.com/doi/10.1002/prp2.109/full

2014
In vitro investigation of silica nanoparticle uptake into human endothelial cells under physiological cyclic stretch.
Freese C, Schreiner D, Anspach L, Bantz C, Maskos M, Unger RE and Kirkpatrick CJ.
Particle and Fibre Toxicology 2014, 11 :1.
http://www.particleandfibretoxicology.com/content/11/1/68/abstract

A novel blood-brain barrier co-culture system for drug targeting of Alzheimer's disease: establishment by using acitretin as a model drug.
Freese, C.; Reinhardt, S., Hefner, G., Unger, R. E., Kirkpatrick, C. J., Endres, K.
PlosOne. e91003.
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0091003

Human endothelial cell-based assay for endotoxin as sensitive as the conventional Limulus Amebocyte Lysate assay.
Unger, R. E.; Peters, K; Sartoris, A; Freese, C; Kirkpatrick, C. J., (2014):
Biomaterials 35(10): 3180-3187.
http://dx.doi.org/10.1016/j.biomaterials.2013.12.059.

2013
Uptake of poly(hydroxypropylamine)-coated gold nanoparticles in microvascular endothelial cells and the transport across the blood-brain barrier.
Freese, C; Brochhausen, C.; Deller, R. C.; Gibson, M. I.; Unger, R. E.; Klok, H.-A; Kirkpatrick, C. J.
Biomater. Sci. 2013, 1 (8), 824 – 833.
http://pubs.rsc.org/en/content/articlelanding/2013/bm/c3bm60050e#!divAbstract.

Design and physicochemical characterization of poly(amidoamine) nanoparticles and the toxicological evaluation in human endothelial cells: applications to peptide delivery to the brain.
Coué G, Freese C, Unger RE, Kirkpatrick CJ, Pickl KE, Sinner FM, Engbersen JF.
J Biomater Sci Polym Ed. 2013;24(8):957-71. doi: 10.1080/09205063.2012.727378. Epub 2012 Oct 2.ct 2.

Bioresponsive poly(amidoamine)s designed for intracellular protein delivery.
Coué G, Freese C, Unger RE, Kirkpatrick CJ, Engbersen JF.
Acta Biomater. 2013 Apr;9(4):6062-74. doi: 10.1016/j.actbio.2012.12.005. Epub 2012 Dec 11.

2012 - 2009
Uptake and cytotoxicity of citrate-coated gold nanospheres: Comparative studies on human endothelial and epithelial cells. HIGHLY ACCESSED
Freese C, Uboldi C, Gibson MI, Unger RE, Weksler BB, Romero IA, Couraud PO, Kirkpatrick CJ.
Part Fibre Toxicol. 2012 Jul 3;9:23. doi: 10.1186/1743-8977-9-23.
http://www.particleandfibretoxicology.com/content/11/1/68/abstract

Size- and coating-dependent uptake of polymer-coated gold nanoparticles in primary human dermal microvascular endothelial cells.
Freese C, Gibson MI, Klok HA, Unger RE, Kirkpatrick CJ.
Biomacromolecules. 2012 May 14;13(5):1533-43. doi: 10.1021/bm300248u. Epub 2012 Apr 18.
http://pubs.acs.org/doi/abs/10.1021/bm300248u

The effects of alpha-secretase ADAM10 on the proteolysis of neuregulin-1.
Freese C, Garratt AN, Fahrenholz F, Endres K.
FEBS J. 2009 Mar;276(6):1568-80. doi: 10.1111/j.1742-4658.2009.06889.x. Epub 2009 Feb 11.