Institute for Molecular Medicine Mainz

Download protocols as PDF files.

CD4cre PCR / IL17Fcre PCR / LCKcre PCR (Pdf, 55,8 KB) DTA PCR / EYFP PCR / eGFP PCR / Dereg PCR (Pdf, 46,2 KB) IFNgR2 PCR (Pdf, 81,9 KB) IL6Ralpha PCR / RORgt PCR (Pdf, 51,5 KB) Rosa26 PCR / IL17F-RFP PCR / IL17RA PCR (Pdf, 105,5 KB) Universal cre PCR / CD11c cre PCR (Pdf, 45,0 KB) Various protocols: Retention of EYFP / EGFP / RFP while staining for FoxP3 / T-bet / RORgammat (Heinen et al Cytometry A 2014)
EAE scoring with pictures  (Pdf, 546,5 KB) Preparation of DNA (Pdf, 67,4 KB) Lymphocytes and Microglia isolation SOP (Pdf, 94,3 KB) 4% PFA for fixation 10x ACK (Pdf, 46,5 KB) Intracellular staining protocol (Pdf, 76,2 KB)