Institute for Molecular Medicine Mainz

Download protocols as PDF files.

CD4cre PCR / IL17Fcre PCR / LCKcre PCR (PDF 55,8 KB)
DTA PCR / EYFP PCR / eGFP PCR / Dereg PCR (PDF 46,2 KB)
IFNgR2 PCR (PDF 81,9 KB)
IL6Ralpha PCR / RORgt PCR (PDF 51,5 KB)
Rosa26 PCR / IL17F-RFP PCR / IL17RA PCR (PDF 105,5 KB)
Universal cre PCR / CD11c cre PCR (PDF 45,0 KB)
Various protocols:
Retention of EYFP / EGFP / RFP while staining for FoxP3 / T-bet / RORgammat (Heinen et al Cytometry A 2014)
EAE scoring with pictures  (PDF 546,5 KB)
Preparation of DNA (PDF 67,4 KB)
Lymphocytes and Microglia isolation SOP (PDF 94,3 KB)
4% PFA for fixation
10x ACK (PDF 46,5 KB)
Intracellular staining protocol (PDF 76,2 KB)