Download protocols as PDF files.

CD4cre PCR / IL17Fcre PCR / LCKcre PCR(PDF 55,8 KB)

DTA PCR / EYFP PCR / eGFP PCR / Dereg PCR(PDF 46,2 KB)

IFNgR2 PCR(PDF 81,9 KB)

IL6Ralpha PCR / RORgt PCR(PDF 51,5 KB)

Rosa26 PCR / IL17F-RFP PCR / IL17RA PCR(PDF 105,5 KB)

Universal cre PCR / CD11c cre PCR(PDF 45,0 KB)

Various protocols:

Retention of EYFP / EGFP / RFP while staining for FoxP3 / T-bet / RORgammat (Heinen et al Cytometry A 2014)

EAE scoring with pictures (PDF 546,5 KB)

Preparation of DNA(PDF 67,4 KB)

Lymphocytes and Microglia isolation SOP(PDF 94,3 KB)

4% PFA for fixation

10x ACK(PDF 46,5 KB)

Intracellular staining protocol(PDF 76,2 KB)