Institute for Molecular Medicine Mainz

Download protocols as PDF files.

CD4cre PCR / IL17Fcre PCR / LCKcre PCR (Pdf-file, 55,8 KB)

DTA PCR / EYFP PCR / eGFP PCR / Dereg PCR (Pdf-file, 46,2 KB)

IFNgR2 PCR (Pdf-file, 81,9 KB)

IL6Ralpha PCR / RORgt PCR (Pdf-file, 51,5 KB)

Rosa26 PCR / IL17F-RFP PCR / IL17RA PCR (Pdf-file, 105,5 KB)

Universal cre PCR / CD11c cre PCR (Pdf-file, 45,0 KB)

Various protocols:

Retention of EYFP / EGFP / RFP while staining for FoxP3 / T-bet / RORgammat (Heinen et al Cytometry A 2014)

EAE scoring with pictures  (Pdf-file, 546,5 KB)

Preparation of DNA (Pdf-file, 67,4 KB)

Lymphocytes and Microglia isolation SOP (Pdf-file, 94,3 KB)

4% PFA for fixation

10x ACK (Pdf-file, 46,5 KB)

Intracellular staining protocol (Pdf-file, 76,2 KB)