International Medical Services
Techniques
In vitro
- Generation of peptide-specific CD8 T cell lines (CTLL)
- Isolation of lymphocytes from different organs (spleen, lymph node, lungs, thymus, bone marrow, liver)
- Immunomagnetical cell separation
- IFN-y based ELISPOT assay
- 51Cr-release assay
- HPLC fractionation
- Cytofluorometric analyses (incl. multimer staining, CFSE analysis)
- Isolation and quantification of endogenously processed peptides from cell and organ extracts
- Quantification of infectious virus in organs
In vivo (mouse model)
- Preemptive cytoimmunotherapy of acute mCMV infection with ex vivo isolated CD8 T cells as well as with in vitro cultivated CTLL (adoptive T cell transfer)
- Experimental bone marrow transplantation (syngeneic, allogeneic)
- In vivo killing of peptide loaded target cells