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Transport and storage of microbiological patient samples
Blood
Material | Transport containers | Storage / Transport |
|---|---|---|
Adults: 8 - 10 ml | Blood culture bottles Bactec aerobic+anaerobic | maximum 24 h at room temperature; specify time of collection! |
Children: 0.5 - 5 ml | Bactec Peds | maximum 24 h at room temperature; specify time of collection! |
Blood for antibody analysis | Serum monovette | cool, 4 - 10°C, transport in 24 h |
Blood for parasite detection | EDTA-Monovette | Direct transport |
Catheter tips
Material | Transport containers | Storage / Transport |
|---|---|---|
Catheter tips | Sterile tube | Direct transport |
CNS and bone marrow
Material | Transport containers | Storage / Transport |
|---|---|---|
Throat, tonsils, nasal swab | Aerobic universal medium | max. 24 h at 4 - 10°C |
Laryngeal epiglottis swab | Aerobic universal medium with thin swab (orange) | Direct transport, max. 2 h at room temperature |
Nasal sinus secretion | Put the dotted material on the Port-a-cul conveyor | max. 12 h at room temperature |
Upper respiratory tract
Material | Transport containers | Storage / Transport |
|---|---|---|
Laryngeal epiglottis swab | Aerobic universal medium with thin swab (orange) | Direct transport, max. 2 h at room temperature |
Nasal sinus secretion | Punctured material on the Port-a-cul conveyor | max. 12 h at room temperature |
Lower airways, lungs
Material | Transport containers | Storage / Transport |
|---|---|---|
Sputum, tracheal bronchial secretion 3 - 5 ml | Sterile sputum tubes | Direct transport, max. 2 h at room temperature |
Pleural fluid | see "sterile body cavities |
|
Biopsy material
Material | Transport containers | Storage / Transport |
|---|---|---|
Biopsy material | Punktured material on the surface of the Port-a-cul | Direct transport |
Ear
Material | Transport container | Storage / Transport |
|---|---|---|
Middle ear secretion with V.a. Otitis media | Punctured material on the surface of the Port-a-cul | max. 12 h at room temperature |
Auditory canal swab | Aerobic universal medium with thin swab | max. 24 h at room temperature |
Eye
Material | Transport container | Storage / Transport |
|---|---|---|
Conjunctival swab | aerobic universal medium with swab | few hours at room temperature |
For chlamydia | special swab instruments | max. 48 h; at 2 - 8°C |
Cell material | Put material on the surface of the Port-a-cul | direct transport |
Vitreous body fluid | see "sterile body cavities" |
|
Sterile body cavities
Material | Transport container | Storage / Transport |
|---|---|---|
Punctates, e.g. pleura, ascites, vitreous fluid | Put as much material as possible (5 - 10 ml) into a sterile tube (black screw cap) Outside the acceptance time: add 1 - 2 ml of material to agar of the Port-a-cul tube and inoculate an aerobic + anaerobic blood culture bottle | Direct transport |
Skin/ Soft tissue
Material | Transport container | Storage / Transport |
|---|---|---|
Exudates (closed process) preferably 2 ml | Put material on the surface of the Port-a-cul | max. 12 h at room temperature |
Exudates (open process) if possible 2 ml, wound swabs | Universal aerobic medium or Port-a-cul tube for deep wounds (anaerobic) | max. 24 h at room temperature |
pieces of tissue | Put material on the surface of the Port-a-cul | direct transport |
Genital tract
Material | Transport container | Storage / Transport |
|---|---|---|
Vaginal, cervical, urethral swab (for bacteria, fungi) | Aerobic universal medium with swab | max. 24 h at 4 - 10°C |
Cervical Urethral Swab (for Chlamydia) | Special swab instruments for Chlamydia. Here you find instructions for correct sampling | max. 48 h at 4 - 10°C |
Vaginal, cervical, urethral swab (for gonococcus) | Transport medium black | Direct transport |
Endometrium | Put the protected swab into Port-a-cul tubes | Direct transport |
Prostatic secretion, ejaculate (2ml) | Place material on the surface of the Port-a-cul tube | max. 4 h at room temperature |
Upper digestive tract
Material | Transport container | Storage / Transport |
|---|---|---|
Gastric biopsy for Helicobacter | Place pieces of tissue on the surface of the Port-a-cul tube | Direct transport |
Esophageal swab | Aerobic universal medium with swab | max. 48 h at 4 - 10°C |
Rinsing liquid on fungi | Universal medium | max. 24 h at 4 - 10°C |
Lower digestive tract
Material | Transport container | Storage / Transport |
|---|---|---|
Stool on bacteria, fungi, EHEC | Cary-Blair stool transport tubes (yellow label) | max. 48 h at 4 - 10°C |
Stool on worm eggs | SAF stool tubes (green label) | max. 24 h at 4 - 10°C |
Stool on oxyurs | Tesa adhesive strips on slides | Transport in a few hours, sample cooling |
Stool on protozoa (antigen detection) | Stool tubes without transport medium (white label) | max. 48 h at 4 - 10°C |
Stool for toxin detection (Clostridium difficile or perfringens) | Stool tubes without transport medium (white label) | max. 24 h at 4 - 10°C |
Urine
Material | Transport container | Storage / Transport |
|---|---|---|
Midstream urine, catheter urine, bladder puncture urine | Add 10 ml to urine monovette (yellow monovette) | cool, few hours storage possible |