Cardiometabolic Function

This platform offers real-time metabolic phenotyping both in rodent and in cell-based models.

Metabolic phenotyping in mice: non-invasive real-time high-resolution behavioral and metabolic phenotyping for rodents. Real-time data acquisition and analysis enables researchers to investigate the abnormal changes in metabolism (food/water intake, energy expenditure and respiratory exchange rate) and behavior (activity/locomotion) of individual animals in physiological and disease settings with a special focus on cardiometabolic diseases.

Metabolic phenotyping in cells: the Seahorse system from Agilent offers real-time measurement of cellular bioenergetics in cells. The 96 well-format seahorse system allows to measure the glycolytic and mitochondrial function of the cells. The system can be adapted to wide range of primary cells derived from genetically modified and disease models as well as tumor cell lines. Specific substrate utilization or fuel-dependency for ATP generation through glycolysis or oxidative phosphorylation can be investigated.

Research groups utilizing these tools

Research Groups at the CTH

• Univ.-Prof. Dr. Christoph Reinhardt (Experimental Hamostasis and Gnotobiotik)
• Univ.-Prof. Dr. Philip Wild (Preventive Cardiology und Medical Prevention,
  Clinical Epidemiology and Systems Medicine)
• Univ.-Prof. Dr. Wven Danckwardt (Experimental Hamostasis and Laborotary Medicine)
• Prof. Dr. Philip Wenzel (Vascular Inflammation)

Platform

• This Platform is part ot the DZHK 'Shared Expertise' ('Plattformlabor CTH' - shared expertise SE076)

Scientific Platform Coordinator

References

• Gogiraju R, Witzler C, Shahneh F, Hubert A, Renner L, Bochenek ML, Zifkos K, Becker C, Thati M, Schäfer K. Deletion of endothelial leptin receptors in mice promotes diet-induced obesity. Sci Rep. 2023 May 22;13(1):8276.