Platform 2

Cardiovascular Function

Cardiovascular diseases (CVD) like myocardial infarction, venous thromboembolism and arterial hypertension with its end organ damages are the leading causes of mortality and morbidity world wide. As one of its central aims, CTH is dedicated to investigate the roles of inflammation and coagulation in the pathogenesis of CVD. The platform lab „cardiovascular function“ comprises mouse models of arterial hypertension (angiotensin II infusion), of venous and arterial thrombosis (vena cava inferior ligation, LAD ligation for induction of myocardial infarction and ischemia-reperfusion-injury), high frequency ultrasound imaging of vasculature and cardiac function (visual sonics, vevo 770) in B-mode, M-Mode and Doppler mode and functional analysis of vascular endothelial and smooth muscle function (Isometric tension studies, AD instruments organ bath and powerlab).

Projects, where the method is used

• CTH Virchow Fellowship Brandt, Molitor
• CTH Doctoral candidate Hanhan Hu
• CTH AvH Professorship Wolfram Ruf
• DZHK doctoral candidate Michael Molitor
• DFG WE 4361/3-1 und WE 4361/4-1
• This platform is also part of the "Platform lab CTH" of the DZHK (shared expertise SE076), www.dzhk.de

References (selection)

• Lagrange J, Kossmann S, Kiouptsi K, Wenzel P (2018) Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls. J Vis Exp. 2018 Jan 4;(131).
• Luther N, Shahneh F, Brähler M, Krebs F, Jäckel S, Subramaniam S, Stanger C, Schönfelder T, Kleis-Fischer B, Reinhardt C, Probst HC, Wenzel P, Schäfer K, Becker C (2016) Innate Effector-Memory T-Cell Activation Regulates Post-Thrombotic Vein Wall Inflammation and Thrombus Resolution. Circ Res 119: 1286-1295.
• Sag CM, Schnelle M, Zhang J, Murdoch CE, Kossmann S, Protti A, Santos CXC, Sawyer G, Zhang X, Mongue-Din H, Richards DA, Brewer AC, Prysyazhna O, Maier LS, Wenzel P, Eaton PJ, Shah AM (2017) Distinct Regulatory Effects of Myeloid Cell and Endothelial Cell NAPDH Oxidase 2 on Blood Pressure. Circulation 135: 2163-2177.
• Subramaniam S, Jurk K, Hobohm L, Jäckel S, Saffarzadeh M, Schwierczek K, Wenzel P, Langer F, Reinhardt C, Ruf W (2017) Distinct contributions of complement factors to platelet activation and fibrin formation in venous thrombus development. Blood 129: 2291-2302.
• Hinkel R, Lange P, Petersen B, Gottlieb E, Ng JK, Finger S, Horstkotte J, Lee S, Thormann M, Knorr M, El-Aouni C, Boekstegers P, Reichart B, Wenzel P, Niemann H, Kupatt C. Heme Oxygenase-1 Gene Therapy Provides Cardioprotection Via Control of Post-Ischemic Inflammation: An Experimental Study in a Pre-Clinical Pig Model. Journal of the American College of Cardiology. 66:154-165, 2015.
• Endothelial p53 Deletion Improves Angiogenesis and Prevents Cardiac Fibrosis and Heart Failure Induced by Pressure Overload in Mice. Gogiraju R, Xu X, Bochenek ML, Steinbrecher JH, Lehnart SE, Wenzel P, Kessel M, Zeisberg EM, Dobbelstein M, Schäfer K. J Am Heart Assoc. pii: e001770. doi: 10.1161/JAHA.115.001770. 2015.
• Henninger C, Huelsenbeck S, Wenzel P, Brand M, Huelsenbeck J, Schad A, Fritz G. Chronic heart damage following doxorubicin treatment is alleviated by lovastatin. Pharmacological research. 91:47-56, 2015.
• Torzewski M, Wenzel P, Kleinert H, Becker C, El-Masri J, Wiese E, Brandt M, Pautz A, Twardowski L, Schmitt E, Munzel T, Reifenberg K. Chronic inflammatory cardiomyopathy of interferon gamma-overexpressing transgenic mice is mediated by tumor necrosis factor-alpha. The American journal of pathology. 180:73-81, 2012.

Scientific Platform-Coordinator

Platform-Supervisor