Platform 1

Intravital Microscopy

Arterial thrombosis is caused by injury of the vascular endothelium or by rupture of atherosclerotic plaques. Consequences can be myocardial infarction or stroke, these are major causes of death worldwide.

One main cause of venous thrombosis is a reduction of blood flow caused by immobility after surgery or during long flights and train journeys. Blood clots often develop in the deep vein legs (deep venous thrombosis, DVT). These clots can break off from vein walls and travel to the lungs, where they can cause a pulmonary embolism.

Projects, where the method is used

• Professorship Ruf (Alexander von Humboldt-Foundation)
• Prof. Dr. Philip Wenzel Vascular Biology
• JG Dr. Markus Bosmann Experimental Research
• Jun.-Prof. Dr. Christoph Reinhardt Translational Research 
• Department of Medicine 2, University Medical Center Mainz, Molecular Cardiology, Prof. Andreas Daiber
• Department of Pharmacology, University Medical Center Mainz, Molecular Pharmacology, Immunopharmacology, Prof. Hartmut Kleinert
• Department of Dermatology, University Medical Center Mainz, PD Dr. Christian Becker
• This platform is also part of the "Platform lab CTH" of the DZHK (shared expertise SE076), www.dzhk.de

Scientific Platform Coordinator

Publications

• Lagrange J, Kossmann S, Kiouptsi K, Wenzel P (2018) Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls. J Vis Exp. 2018 Jan 4;(131).
• Schönfelder T, Jäckel S, Wenzel P (2017) Mouse models of deep vein thrombosis. Gefasschirurgie. 2017;22(Suppl 1):28-33.
• Kossmann S, Lagrange J, Jäckel S, Jurk K, Ehlken M, Schönfelder T, Weihert Y, Knorr M, Brandt M, Xia N, Li H, Daiber A, Oelze M, Reinhardt C, Lackner K, Gruber A, Monia B, Karbach SH, Walter U, Ruggeri ZM, Renné T, Ruf W, Münzel T, Wenzel P (2017) Platelet-localized FXI promotes a vascular coagulation-inflammatory circuit in arterial hypertension. Sci Transl Med 9.
• Busch CJ, Hendrikx T, Weismann D, Jäckel S, Walenbergh SM, Rendeiro AF, Weisser J, Puhm F, Hladik A, Göderle L, Papac-Milicevic N, Haas G, Millischer V, Subramaniam S, Knapp S, Bennett KL, Bock C, Reinhardt C, Shiri-Sverdlov R, Binder CJ (2017) Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice. Hepatology 65: 1181-1195.
• Müller-Calleja N, Manukyan D, Canisius A, Strand D, Lackner KJ (2017) Hydroxychloroquine inhibits proinflammatory signalling pathways by targeting endosomal NADPH oxidase. Ann Rheum Dis 76: 891-897.
• Luther N, Shahneh F, Brähler M, Krebs F, Jäckel S, Subramaniam S, Stanger C, Schönfelder T, Kleis-Fischer B, Reinhardt C, Probst HC, Wenzel P, Schäfer K, Becker C (2016) Innate Effector-Memory T-Cell Activation Regulates Post-Thrombotic Vein Wall Inflammation and Thrombus Resolution. Circ Res 119: 1286-1295.
• Karbach SH, Schönfelder T, Brandao I, Wilms E, Hörmann N, Jäckel S, Schüler R, Finger S, Knorr M, Lagrange J, Brandt M, Waisman A, Kossmann S, Schäfer K, Münzel T, Reinhardt C, Wenzel P (2016) Gut Microbiota Promote Angiotensin II-Induced Arterial Hypertension and Vascular Dysfunction. Journal of the American Heart Association 5.
• Manukyan D, Müller-Calleja N, Jäckel S, Luchmann K, Monnikes R, Kiouptsi K, Reinhardt C, Jurk K, Walter U, Lackner KJ. Cofactor Independent Human Antiphospholipid Antibodies Induce Venous Thrombosis in Mice. Journal of thrombosis and haemostasis. 2016.
• Wenzel P, Rossmann H, Müller C, Kossmann S, Oelze M, Schulz A, Arnold N, Simsek C, Lagrange J, Klemz R, Schönfelder T, Brandt M, Karbach SH, Knorr M, Finger S, Neukirch C, Häuser F, Beutel ME, Kröller-Schön S, Schulz E, Schnabel RB, Lackner K, Wild PS, Zeller T, Daiber A, Blankenberg S, Münzel T. Heme oxygenase-1 suppresses a pro-inflammatory phenotype in monocytes and determines endothelial function and arterial hypertension in mice and humans. European heart journal 36:3437-3446, 2015.
• Bollmann F*, Jäckel S*, Schmidtke L, Schrick K, Reinhardt C, Jurk K, Wu Z, Xia N, Li H, Erkel G, Walter U, Kleinert H**, Pautz A**. Anti-Inflammatory and Anti-Thrombotic Effects of the Fungal Metabolite Galiellalactone in Apolipoprotein E-Deficient Mice. PloS one. 10:e0130401, 2015.
  * These authors contributed equally to this work.
  ** AP and HK are co-senior authors.
• Schönfelder T, Jäckel S, Wenzel P. Mouse models of deep vein thrombosis. Gefässchirurgie. 19:257–262, 2014.