Introduction to RNA-Seq and NGS

Within this course, a basic introduction of RNA sequencing will be provided, covering bulk RNA-Seq for gene expression analysis, as well as single cell RNA-Seq and fusion gene detection. We will detail the workflow from RNA sample to analyzed data and give practical examples of data analysis and visualization.

Organizer and trainers from: TRON, University Medical Center Mainz

Topics:

• Introduction to RNA-Seq and molecular biology background
  • sample preparation, library preparation
  • sequencing (Illumina)
  • computation of expression values
  • differential expression & functional analysis
  • application example
  • Q&A

• Single Cell RNA-Seq and Spatial Transcriptomics 
  
  • sample preparation, library preparation
  • differences to bulk RNA-seq
  • data analysis
  • application example
  • Q&A

• Fusion gene detection
  • overview
  • principle of detection from NGS data
  • application example
  • Q&A

Methodology: Theoretical and practical input by the trainer by showing examples.

Target audience: TransMed Fellows (doctoral candidates, postdocs and clinician scientists)

Maximum number of participants: 30

Next workshop: TBA

Venue: TBA
 

For the workshop 0.5 CP for scientific skills training can be credited.