Introduction to RNA-Seq and NGS

Within this course, a basic introduction of RNA sequencing will be provided, covering bulk RNA-Seq for gene expression analysis, as well as single cell RNA-Seq and fusion gene detection. We will detail the workflow from RNA sample to analyzed data and give practical examples of data analysis and visualization.

Organizer and trainers from: TRON, University Medical Center Mainz

Topics:

• Introduction to RNA-Seq and molecular biology background
  • sample preparation, library preparation
  • sequencing (Illumina)
  • computation of expression values
  • differential expression & functional analysis
  • application example
  • Q&A
• Single Cell RNA-Seq and Spatial Transcriptomics 
  • sample preparation, library preparation
  • differences to bulk RNA-seq
  • data analysis
  • application example
  • Q&A
• Fusion gene detection
  • overview
  • principle of detection from NGS data
  • application example
  • Q&A

Methodology: Theoretical and practical input by the trainer by showing examples.

Target audience: All TransMed Fellows (early career researchers)

Maximum number of participants: 30

Next workshop: March 10, 9.00 a.m. - 1.00 p.m., 2026

Venue: Seminar Room 2 in Building 708, University Medical Center Mainz 
 
For the workshop, 0.5 TransMed CP for scientific skills training can be credited.