Visual Universitätsmedizin Mainz

Introduction to RNA-Seq and NGS

Within this online course, a basic introduction of RNA sequencing will be provided, covering bulk RNA-Seq for gene expression analysis, as well as single cell RNA-Seq and fusion gene detection. We will detail the workflow from RNA sample to analyzed data and give practical examples of data analysis and visualization.

Organizer and trainers from: TRON, University Medical Center Mainz


  • Introduction to RNA-Seq and molecular biology background

    • sample preparation, library preparation
    • sequencing (Illumina)
    • computation of expression values
    • differential expression & functional analysis
    • application example
    • Q&A

  • Single Cell RNA-Seq

    • sample preparation, library preparation
    • differences to bulk RNA-seq
    • data analysis
    • application example
    • Q&A

  • Fusion gene detection

    • overview
    • principle of detection from NGS data
    • application example
    • Q&A

Methodology: Theoretical and practical input by the trainer by showing examples.

Target audience: TransMed Fellows (doctoral candidates, postdocs and clinician scientists)

Maximum number of participants: unlimited

Next workshop: to be announced 

Technology: Zoom
Please check that your PC meets the requirements beforehand (microphone, camera, Internet connection).  

For the workshop 0.5 CP for scientific skills training can be credited.


Ivonne Dietzel
Program Coordinator
Bldg. 907, Room 01-212
University Medical Center
Langenbeckstr. 1
55131 Mainz

Tel. +49 6131 17-7631