The flow of information in the central nervous system is critically shaped by the molecular architecture of synapses, which contain a vast protein machinery that determines the properties of synaptic transmission. Alterations in the synaptic proteome have been linked to a wide range of neurological and psychiatric disorders, and quantifying such changes is therefore essential for understanding the molecular mechanisms underlying these disorders. However, this process is rendered challenging by to a number of factors, including the generally subtle nature of the molecular alterations, the diversity of synapse subtypes, and technical limitations in accurately identifying the synaptic protein machinery. In this one-day workshop, we will discuss biochemical, imaging, and proteomic methodologies for the study of synaptic proteins, as well as exploring analysis strategies that facilitate the quantification of subtle or synapse-specific molecular changes. In addition, we will demonstrate immunohistochemical and image analysis approaches toward quantifying the synaptic proteome, with a focus on inhibitory synapses. Overall, this workshop will assist students in designing their own experiments to quantify the synaptic proteome, as well as enabling them to better evaluate the relevant literature.
Lecturer: Prof. Dilja Krueger-Burg, Institute of Anatomy, University Medical Center Mainz
Target audience: TransMed's doctoral students, postdocs, and clinician scientists
Maximum number of participants: 8
Next workshop: TBA
Venue: Institute of Anatomy, Biomedizinisches Forschungszentrum (BFZ), Duesbergweg 6, Room 00.321, UMC Mainz
For the workshop, 0.8 CP for scientific skills training can be credited.