FlowJoTM Advanced Workshop

Workshop description

This beyond the basic workshop shows participants how to analyze flow cytometry data using FlowJo^TM.  In the morning, the software is explained theoretically using examples. In the afternoon, participants practice FlowJo in pairs using own data. There is enough time to discuss specific issues.

Topics:

Workflow:

• Introduction & help resources
• General overview of FlowJo^TM including the following:
  • Loading samples
  • Sample quality check & time gates
  • Keywords and groups
  • Gating
  • Adding statistics
  • Creating and exporting tables
  • Creating a layout and batching
  • Overlays - grouping by panel or keyword
  • Overlays - compare against a control
  • Heat mapping statistics in the Table and Layout Editors
  • Saving your work

For "power users":

• Compensation
• Creating a compensation matrix using the Wizard
• Editing an existing compensation matrix
• Saving compensation matrices
• Deleting compensation matrices
• Cell cycle
• Proliferation
• Concatenation and export
• Creating templates
• Using R-dependent plugins with FlowJo^TM
• Using R-independent plugins with FlowJo^TM
• PCA/t-SNE
• SPADE/FlowSOM

Methodology:
Theoretical and practical input by the trainer, showing examples, analyzing own data using FlowJo^TM with the help of the trainer, discussing own issues, feedback from the trainer.

Target audience, number of participants:
Doctoral students, postdocs and clinician scientists who already know the basics of FlowJo^TM.

Maximum number of participants: 14

Preparation:
Please read the FlowJo^TM description, which will be sent to you after your registration. Please bring your own data with you in order to analyze them.

Next workshop: to be announced

For the workshop 1 CP for scientific skills training can be credited (0.5 CP for participating only in the theoretical part).

This workshop is co-organized by TransMed and the Core Facility Flow Cytometry (CFFC):

About the Core Facility Flow Cytometry (CFFC):

Everyone is welcome to use FlowJo^TM on the CFFC workstations, currently free of charge.
If you are sorting in the CFFC you will receive step-by-step tuition and guidance leading you to being able to work with the FACSAria sorter independently and operator-free. This service is currently free of charge, too.