Mass Spectrometry-based Metabolomics: Technology and Applications
Trainers: Jun. Prof. Dr. Thierry Schmidlin, Dr. Boyao Zhang; Institute of Immunology, University Medical Center Mainz
Topics: - Introduction
- The clinical relevance of measuring small molecules
- Typical compound coverage achieved by metabolomics, and their significance: e.g. endogenous metabolites (energy balance, anabolism/catabolism, signaling), exogenous compounds (ADME, xenobiotics, antigens)
- Typical entry points of metabolomics in biochemical/medical research
- Examples of successful metabolomics applications
- Basics of MS based Metobolomics
- Introduction to LC-MS/MS technology
- Levels of information obtained from chromatography, MS and MS/MS
- Strategies to infer chemical/biological information from these datasets
- Planning an experiment
- Strategies for successful experiment design (targeted metabolomics, metabolic profiling, flux analysis)
- Dos and don'ts in MS sample preparation
- Key aspects to be considered such as analyte stability, measurement sensitivity, preanalytical consideration, best extraction procedures, compatibility of metabolomics with various experiment- and sample types (e.g. FACS, FFPE)
- Data analysis and interpretation
- Basic data analysis strategies: how to best proceed after obtaining a dataset
- Distilling biological information from MS data and designing follow up experiments
- Q&A
Methodology: Lectures and practical demonstrations in two small working groups
Target audience: Doctoral candidates, clinician scientists, postdoctoral researchers
Preparation: There is room to cover specific additional topics if required – please send your requests to TransMed two weeks in advance.
Maximum Number of Participants: 10
Next workshop: TBATransMed Creditpoints: 0,8 in scientific skills