Centrum für Thrombose und Hämostase (CTH)

Visual Universitätsmedizin Mainz

Prof. Dr. Ulrich Walter

(Wissenschaftlicher Direktor CTH bis 09/2014)

Univ.-Prof. Dr. med. Ulrich Walter
Univ.-Prof. Dr. med. Ulrich Walter
Funktionen:

Seniorprofessor CTH, Mitglied der Leopoldina (ML), Nationale Akademie der Wissenschaften


Im Januar 2012 wechselte Prof. Walter vom Uniklinikum Würzburg an das CTH und übernahm die Professur „Translationale Forschung in Thrombose und Hämostase“, die bis September 2014 mit der Funktion des Wissenschaftlichen Direktors des CTH verbunden war. Prof. Walter ist Experte auf dem Gebiet der Thrombose und Hämostase und unterstützt die Entwicklung des CTH als Senior Professor.

Originalarbeiten

  • Zhang P, Solari FA, Heemskerk JWM, Kuijpers MJE, Sickmann A, Walter U, Jurk K. Differential Regulation of GPVI-Induced Btk and Syk Activation by PKC, PKA and PP2A in Human Platelets. Int J Mol Sci. 2023 Apr 24;24(9):7776. doi: 10.3390/ijms24097776

  • Shpakova V, Rukoyatkina N, Walter U, Gambaryan S. Potential and limitations of PKA/ PKG inhibitors for platelet studies. Platelets. 2022 Aug 18;33(6):859-868

  • Kyselova A, Siragusa M, Anthes J, Solari FA, Loroch S, Zahedi RP, Walter U, Fleming I, Randriamboavonjy V. Cyclin Y is expressed in Platelets and Modulates Integrin Outside-in Signaling. Int J Mol Sci. 2020 Nov 3;21(21):823

  • Makhoul S, Kumm E, Zhang P, Walter U, Jurk K. The Serine/Threonine Protein Phosphatase 2A (PP2A) Regulates Syk Activity in Human Platelets. Int J Mol Sci. 2020 Nov 25;21(23):8939

  • Comer S, Nagy Z, Bolado A, von Kriegsheim A, Gambaryan S, Walter U, Pagel O, Zahedi RP, Jurk K, Smolenski A. The RhoA regulators Myo9b and GEF-H1 are targets of cyclic nucleotide-dependent kinases in platelets. J Thromb Haemost. 2020 Nov;18(11):3002-3012

  • Döhrmann M, Makhoul S, Gross K, Krause M, Pillitteri D, von Auer C, Walter U, Lutz J, Volf I, Kehrel BE, Jurk K. CD36-fibrin interaction propagates FXI-dependent thrombin generation of human platelets. FASEB J. 2020 Jul;34(7):9337-9357

  • Kumm EJ, Pagel O, Gambaryan S, Walter U, Zahedi RP, Smolenski A, Jurk K. The Cell Cycle Checkpoint System MAST(L)-ENSA/ARPP19-PP2A is Targeted by cAMP/PKA and cGMP/PKG in Anucleate Human Platelets. Cells. 2020 Feb 18;9(2):472

  • Kiouptsi K, Finger S, Garlapati VS, Knorr M, Brandt M, Walter U, Wenzel P, Reinhardt C. Hypoxia evokes increased PDI and PDIA6 expression in the infarcted myocardium of ex-germ-free and conventionally raised mice. Biol Open 2019;8(1)

  • Kiouptsi K, Jackel S, Pontarollo G, Grill A, Kuijpers MJE, Wilms E, Weber C, Sommer F, Nagy M, Neideck C, Jansen Y, Ascher S, Formes H, Karwot C, Bayer F, Kollar B, Subramaniam S, Molitor M, Wenzel P, Rosenstiel P, Todorov H, Gerber S, Walter U, Jurk K, Heemskerk JWM, van der Vorst EPC, Doring Y and Reinhardt C. The Microbiota Promotes Arterial Thrombosis in Low-Density Lipoprotein Receptor-Deficient Mice. Mbio. 2019;10

  • Makhoul S, Trabold K, Gambaryan S, Tenzer S, Pillitteri D, Walter U and Jurk K. cAMP- and cGMP-elevating agents inhibit GPIb alpha-mediated aggregation but not GPIb alpha-stimulated Syk activation in human platelets. Cell Communication and Signaling. 2019;17

  • Trabold K, Makhoul S, Garnbaryan S, van Ryn J, Walter U and Jurk K. The Direct Thrombin Inhibitors Dabigatran and Lepirudin Inhibit GPIb alpha-Mediated Platelet Aggregation. Thrombosis and Haemostasis. 2019;119:916-929

  • Comer S, Nagy Z, Bolado A, von Kriegsheim A, Gambaryan S, Walter U, Pagel O, Zahedi RP, Jurk K and Smolenski A. The RhoA regulators Myo9b and GEF-H1 are targets of cyclic nucleotide-dependent kinases in platelets. Journal of Thrombosis and Haemostasis. 2020;18:3002-3012

  • Dohmann M, Makhoul S, Gross K, Krause M, Pillitteri D, von Auer C, Walter U, Lutz J, Volf I, Kehrel BE and Jurk K. CD36-fibrin interaction propagates FXI-dependent thrombin generation of human platelets. Faseb Journal

  • Kumm EJ, Pagel O, Gambaryan S, Walter U, Zahedi RP, Smolenski A and Jurk K. The Cell Cycle Checkpoint System MAST(L)-ENSA/ARPP19-PP2A is Targeted by cAMP/PKA and cGMP/PKG in Anucleate Human Platelets. Cells. 2020;9

  • Kyselova A, Siragusa M, Anthes J, Solari FA, Loroch S, Zahedi RP, Walter U, Fleming I and Randriamboavonjy V. Cyclin Y Is Expressed in Platelets and Modulates Integrin Outside-in Signaling. International Journal of Molecular Sciences. 2020;21

  • Makhoul S, Dorschel S, Gambaryan S, Walter U and Jurk K. Feedback Regulation of Syk by Protein Kinase C in Human Platelets. International Journal of Molecular Sciences. 2020;21

  • Makhoul S, Kumm E, Zhang PY, Walter U and Jurk K. The Serine/Threonine Protein Phosphatase 2A (PP2A) Regulates Syk Activity in Human Platelets. International Journal of Molecular Sciences. 2020;21

  • Schönfelder T, Brandt M, Kossmann S, Knopp T, Münzel T, Walter U, Karbach SH, Wenzel P. Lack of T-bet reduces monocytic interleukin-12 formation and accelerates thrombus resolution in deep vein thrombosis. Sci Rep 2018;8(1):3013

  • Makhoul S, Walter E, Pagel O, Walter U, Sickmann A, Gambaryan S, Smolenski A, Zahedi RP, Jurk K. Effects of the NO/soluble guanylate cyclase/cGMP system on the functions of human platelets. Nitric Oxide 2018;76:71-80

  • Beck F, Geiger J, Gambaryan S, Solari FA, Dell'Aica M, Loroch S, Mattheij NJ, Mindukshev I, Potz O, Jurk K, Burkhart JM, Fufezan C, Heemskerk JW, Walter U, Zahedi RP, Sickmann A (2017) Temporal quantitative phosphoproteomics of ADP stimulation reveals novel central nodes in platelet activation and inhibition. Blood 129: e1-e12

  • Jäckel S, Kiouptsi K, Lillich M, Hendrikx T, Khandagale A, Kollar B, Hörmann N, Reiss C, Subramaniam S, Wilms E, Ebner K, Brühl MV, Rausch P, Baines JF, Haberichter S, Lämmle B, Binder CJ, Jurk K, Ruggeri ZM, Massberg S, Walter U, Ruf W, Reinhardt C (2017) Gut microbiota regulate hepatic von Willebrand factor synthesis and arterial thrombus formation via Toll-like receptor-2. Blood 130: 542-553

  • Kiouptsi K, Gambaryan S, Walter E, Walter U, Jurk K, Reinhardt C (2017) Hypoxia impairs agonist-induced integrin alphaIIbbeta3 activation and platelet aggregation. Scientific reports 7: 7621

  • Kossmann S, Lagrange J, Jäckel S, Jurk K, Ehlken M, Schönfelder T, Weihert Y, Knorr M, Brandt M, Xia N, Li H, Daiber A, Oelze M, Reinhardt C, Lackner K, Gruber A, Monia B, Karbach SH, Walter U, Ruggeri ZM, Renné T, Ruf W, Münzel T, Wenzel P (2017) Platelet-localized FXI promotes a vascular coagulation-inflammatory circuit in arterial hypertension. Sci Transl Med 9

  • Loroch S, Trabold K, Gambaryan S, Reiss C, Schwierczek K, Fleming I, Sickmann A, Behnisch W, Zieger B, Zahedi RP, Walter U, Jurk K (2017) Alterations of the platelet proteome in type I Glanzmann thrombasthenia caused by different homozygous delG frameshift mutations in ITGA2B. Thrombosis and haemostasis 117: 556-569

  • Rukoyatkina N, Butt E, Subramanian H, Nikolaev VO, Mindukshev I, Walter U, Gambaryan S, Benz PM (2017) Protein kinase A activation by the anti-cancer drugs ABT-737 and thymoquinone is caspase-3-dependent and correlates with platelet inhibition and apoptosis. Cell Death Dis 8: e2898

  • enz PM, Laban H, Zink J, Günther L, Walter U, Gambaryan S, Dib K (2016) Vasodilator-Stimulated Phosphoprotein (VASP)-dependent and -independent pathways regulate thrombin-induced activation of Rap1b in platelets. Cell Commun Signal 14: 21

  • Gambaryan S, Subramanian H, Kehrer L, Mindukshev I, Sudnitsyna J, Reiss C, Rukoyatkina N, Friebe A, Sharina I, Martin E, Walter U (2016) Erythrocytes do not activate purified and platelet soluble guanylate cyclases even in conditions favourable for NO synthesis. Cell Commun Signal 14: 16

  • Manukyan D, Müller-Calleja N, Jäckel S, Luchmann K, Monnikes R, Kiouptsi K, Reinhardt C, Jurk K, Walter U, Lackner KJ (2016) Cofactor-independent human antiphospholipid antibodies induce venous thrombosis in mice. Journal of thrombosis and haemostasis 14: 1011-20

  • Reiss C, Mindukshev I, Bischoff V, Subramanian H, Kehrer L, Friebe A, Stasch JP, Gambaryan S, Walter U. The sGC stimulator Riociguat inhibits platelet function in washed platelets but not in whole blood. British journal of pharmacology, 2015

  • Bollmann F, Jäckel S, Schmidtke L, Schrick K, Reinhardt C, Jurk K, Wu Z, Xia N, Li H, Erkel G, Walter U, Kleinert H, Pautz A. Anti-inflammatory and anti-thrombotic effects of the fungal metabolite galiellalactone in apolipoprotein e-deficient mice. PLoS One. 2015;10:e013040

  • Frank B, Ariza L, Lamparter H, Grossmann V, Prochaska JH, Ullmann A, Kindler F, Weisser G, Walter U, Lackner KJ, Espinola-Klein C, Münzel T, Konstantinides SV, Wild PS. Rationale and design of three observational, prospective cohort studies including biobanking to evaluate and improve diagnostics, management strategies and risk stratification in venous thromboembolism: The vteval project. BMJ Open. 2015;5:e008157

  • Prochaska JH, Gobel S, Keller K, Coldewey M, Ullmann A, Lamparter H, Junger C, Al-Bayati Z, Baer C, Walter U, Bickel C, Ten Cate H, Munzel T, Wild PS. Quality of oral anticoagulation with phenprocoumon in regular medical care and its potential for improvement in a telemedicine-based coagulation service inverted question mark results from the prospective, multi-center, observational cohort study thrombEVAL. BMC medicine. 13:14, 2015

  • Navdaev A, Subramanian H, Petunin A, Clemetson KJ, Gambaryan S, Walter U. Echicetin coated polystyrene beads: a novel tool to investigate GPIb-specific platelet activation and aggregation. PloS one 9:e93569, 2014

  • Hubertus K, Mischnik M, Timmer J, Herterich S, Mark R, Moulard M, Walter U, Geiger J. Reciprocal regulation of human platelet function by endogenous prostanoids and through multiple prostanoid receptors. European journal of pharmacology. 740C:15-27, 2014

  • Hörmann N, Brandao I, Jäckel S, Ens N, Lillich M, Walter U, Reinhardt C. Gut microbial colonization orchestrates TLR2 expression, signaling and epithelial proliferation in the small intestinal mucosa. PloS one. 9:e113080, 2014

  • Beck F, Geiger J, Gambaryan S, Veit J, Vaudel M, Nollau P, Kohlbacher O, Martens L, Walter U, Sickmann A, Zahedi RP. Time-resolved characterization of camp/pka-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways. Blood. 123:e1-e10, 2014

  • Tenzer S, Docter D, Kuharev J, Musyanovych A, Fetz V, Hecht R, Schlenk F, Fischer D, Kiouptsi K, Reinhardt C, Landfester K, Schild H, Maskos M, Knauer SK, Stauber RH. Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology. Nature nanotechnology. 8:772-781, 2013

  • Subramanian H, Zahedi RP, Sickmann A, Walter U, Gambaryan S. Phosphorylation of caldag-gefi by protein kinase a prevents rap1b activation. J Thromb Haemost. 11:1574-1582, 2013.

  • Schweigel H, Geiger J, Beck F, Buhs S, Gerull H, Walter U, Sickmann A, Nollau P. Deciphering of adp-induced, phosphotyrosine-dependent signaling networks in human platelets by src-homology 2 region (sh2)-profiling. Proteomics. 13:1016-1027, 2013

  • Rukoyatkina N, Mindukshev I, Walter U, Gambaryan S. Dual role of the p38 mapk/cpla(2) pathway in the regulation of platelet apoptosis induced by abt-737 and strong platelet agonists. Cell Death Dis. 4, 2013

  • Mihlan S, Reiss C, Thalheimer P, Herterich S, Gaetzner S, Kremerskothen J, Pavenstadt HJ, Lewandrowski U, Sickmann A, Butt E. Nuclear import of lasp-1 is regulated by phosphorylation and dynamic protein-protein interactions. Oncogene. 32:2107-2113, 2013

  • Hausding M, Jurk K, Daub S, Kroller-Schon S, Stein J, Schwenk M, Oelze M, Mikhed Y, Kerahrodi JG, Kossmann S, Jansen T, Schulz E, Wenzel P, Reske-Kunz AB, Becker C, Munzel T, Grabbe S, Daiber A. Cd40l contributes to angiotensin ii-induced pro-thrombotic state, vascular inflammation, oxidative stress and endothelial dysfunction. Basic research in cardiology. 108:386, 2013

  • Hahne JC, Meyer SR, Gambaryan S, Walter U, Dietl J, Engel JB, Honig A. Immune escape of akt overexpressing ovarian cancer cells. Int J Oncol. 42:1630-1635, 2013

  • Grunewald TG, Willier S, Janik D, Unland R, Reiss C, da Costa OP, Buch T, Dirksen U, Richter GH, Neff F, Burdach S, Butt E. The zyxin-related protein thyroid receptor interacting protein 6 (trip6) is overexpressed in ewing's sarcoma and promotes migration, invasion and cell growth. Biology of the cell / under the auspices of the European Cell Biology Organization. 2013

  • Geiger J, Burkhart JM, Gambaryan S, Walter U, Sickmann A, Zahedi RP. Response: Platelet transcriptome and proteome--relation rather than correlation. Blood. 121:5257-5258, 2013

  • Gambaryan S, Subramanian H, Rukoyatkina N, Herterich S, Walter U. Soluble guanylyl cyclase is the only enzyme responsible for cyclic guanosine monophosphate synthesis in human platelets. Thromb Haemostasis. 109:973-975, 2013

  • Fehrmann C, Jurk K, Bertling A, Seidel G, Fegeler W, Kehrel BE, Peters G, Becker K, Heilmann C. Role for the fibrinogen-binding proteins coagulase and efb in the staphylococcus aureus-candida interaction. International journal of medical microbiology: IJMM. 303:230-238, 2013

  • Erdmann J, Stark K, Esslinger UB, Rumpf PM, Koesling D, de Wit C, Kaiser FJ, Braunholz D, Medack A, Fischer M, Zimmermann ME, Tennstedt S, Graf E, Eck S, Aherrahrou Z, Nahrstaedt J, Willenborg C, Bruse P, Braenne I, Nothen MM, Hofmann P, Braund PS, Mergia E, Reinhard W, Burgdorf C, Schreiber S, Balmforth AJ, Hall AS, Bertram L, Steinhagen-Thiessen E, Li SC, Marz W, Reilly M, Kathiresan S, McPherson R, Walter U, Ott J, Samani NJ, Strom TM, Meitinger T, Hengstenberg C, Schunkert H, Assimes TL, Deloukas P, Holm H, Konig IR, Roberts R, Stewart AF. Dysfunctional nitric oxide signalling increases risk of myocardial infarction. Nature. 504:432-436, 2013

  • Brandt M, Schonfelder T, Schwenk M, Becker C, Jackel S, Reinhardt C, Stark K, Massberg S, Munzel T, von Bruhl ML, Wenzel P. Deep vein thrombus formation induced by flow reduction in mice is determined by venous side branches. Clinical hemorheology and microcirculation. 2013

  • Begonja AJ, Gambaryan S, Schulze H, Patel-Hett S, Italiano JE, Hartwig JH, Walter U. Differential roles of camp and cgmp in megakaryocyte maturation and platelet biogenesis. Exp Hematol. 41:91-101, 2013

  • Anaraki PK, Patecki M, Larmann J, Tkachuk S, Jurk K, Haller H, Theilmeier G, Dumler I. Urokinase receptor mediates osteogenic differentiation of mesenchymal stem cells and vascular calcification via the complement c5a receptor. Stem cells and development. 2013

  • Subramanian H, Gambaryan S, Panzer S, Gremmel T, Walter U, Mannhalter C. The thr715pro variant impairs terminal glycosylation of p-selectin. Thromb Haemostasis. 108:963-972, 2012

  • Sturm JB, Hess M, Weibel S, Chen NG, Yu YA, Zhang Q, Donat U, Reiss C, Gambaryan S, Krohne G, Stritzker J, Szalay AA. Functional hyper-il-6 from vaccinia virus-colonized tumors triggers platelet formation and helps to alleviate toxicity of mitomycin c enhanced virus therapy. Journal of translational medicine. 10:9, 2012

  • Reinhardt C, Bergentall M, Greiner TU, Schaffner F, Ostergren-Lunden G, Petersen LC, Ruf W, Backhed F. Tissue factor and par1 promote microbiota-induced intestinal vascular remodelling. Nature. 483:627-631, 2012

  • Mindukshev I, Gambaryan S, Kehrer L, Schuetz C, Kobsar A, Rukoyatkina N, Nikolaev VO, Krivchenko A, Watson SP, Walter U, Geiger J. Low angle light scattering analysis: A novel quantitative method for functional characterization of human and murine platelet receptors. Clin Chem Lab Med. 50:1253-1262, 2012

  • Kobsar A, Koessler J, Kehrer L, Gambaryan S, Walter U. The thrombin inhibitors hirudin and refludan (r) activate the soluble guanylyl cyclase and the cgmp pathway in washed human platelets. Thromb Haemostasis. 107:521-529, 2012

  • Horn M, Bertling A, Brodde MF, Muller A, Roth J, Van Aken H, Jurk K, Heilmann C, Peters G, Kehrel BE. Human neutrophil alpha-defensins induce formation of fibrinogen and thrombospondin-1 amyloid-like structures and activate platelets via glycoprotein iib/iiia. J Thromb Haemost. 10:647-661, 2012.

  • Hahne JC, Honig A, Meyer SR, Gambaryan S, Walter U, Wischhusen J, Haussler SFM, Segerer SE, Fujita N, Dietl J, Engel JB. Downregulation of akt reverses platinum resistance of human ovarian cancers in vitro. Oncol Rep. 28:2023-2028, 2012.

  • Gambaryan S, Friebe A, Walter U. Does the no/sgc/cgmp/pkg pathway play a stimulatory role in platelets? Blood. 119:5335-5336, 2012.

  • Gambaryan S, Butt E, Kobsar A, Geiger J, Rukoyatkina N, Parnova R, Nikolaev VO, Walter U. The oligopeptide dt-2 is a specific pkg i inhibitor only in vitro, not in living cells. Brit J Pharmacol. 167:826-838, 2012.

  • Caesar R, Reigstad CS, Backhed HK, Reinhardt C, Ketonen M, Lunden GO, Cani PD, Backhed F. Gut-derived lipopolysaccharide augments adipose macrophage accumulation but is not essential for impaired glucose or insulin tolerance in mice. Gut. 61:1701-1707, 2012.

  • Burkhart JM, Vaudel M, Gambaryan S, Radau S, Walter U, Martens L, Geiger J, Sickmann A, Zahedi RP. The first comprehensive and quantitative analysis of human platelet protein composition allows the comparative analysis of structural and functional pathways. Blood. 120:E73-E82, 2012.

  • Bertling A, Niemann S, Hussain M, Holbrook L, Stanley RG, Brodde MF, Pohl S, Schifferdecker T, Roth J, Jurk K, Muller A, Lahav J, Peters G, Heilmann C, Gibbins JM, Kehrel BE. Staphylococcal extracellular adherence protein induces platelet activation by stimulation of thiol isomerases. Arteriosclerosis, thrombosis, and vascular biology. 32:1979-1990, 2012.

 

Reviews

  • Shiravand Y, Walter U, Jurk K. Fine-Tuning of Platelet Responses by Serine/Threonine Protein Kinases and Phosphatases-Just the Beginning. Hamostaseologie. 2021 Jun;41(3):206-216
  • Jurk K, Walter U. New Insights into Platelet Signalling Pathways by Functional and Proteomic Approaches. Hamostaseologie 2018
  • Lämmle B, Walter U. Pearls, guidelines & more. Hamostaseologie. 34:199, 2014
  • Burkhart JM, Gambaryan S, Watson SP, Jurk K, Walter U, Sickmann A, Heemskerk JWM, Zahedi RP. What Can Proteomics Tell Us About Platelets?. Circulation Research. 114:1204-1219, 2014
Informationen zum CTH